ODiate® Excitation Filter, 25.0 mm, 474 nm CWL, 37 nm FWHM

The OD8-EX474-37-B Excitation Filter is specified for excitaiton of GFP and supports similar alternative fluorophores, designed in conjunction with and . The filter is manufactured to provide maximum signal and sensitivity delivering exceptionally high passband transmission ≥ 96% avg for 474 nm CWL and 37 nm FWHM and enhanced blocking ≥ OD10 avg over the corresponding emission passband.

Alternate Fluorophores

In addition to GFB the following fluorophores are also compatible with this set:

5-FAM, Abberior STAR GREEN, Azami Green, Azami Green (monomeric), BCECF@pH5.2, BCECF@pH5.5, C3-Oxacyanine, Calcein, CF 450, CF 488A, CFP2, Coumarin 6, Cy^®2, DiO, Emerald, FITC, Fluo-4, Fluorescein (0.1M NaOH), Fluorescein (IgG), HCS CellMask Green, LysoSensor Green, mAzamiGreen, mEmerald, mHoneydew, MitoSpy Green FM, MitoTracker® Green FM, mWasabi, NBD, NBD-X, NeuroTrace^® 500/525 Green Fluorescent Nissl Stain (RNA), PromoFluor-488, PromoFluor-488 Premium, PS-CFP2, sgGFP, SYBR® Green I, SYTO^® 13, SYTO^® 16, SYTO^® 9, TagGFP, TagGFP2, TurboGFP, WEGFP (post-activation), YO-PRO^®-1, YOYO^®-1.

Optimized Coatings and Engineering Expertise

Newport pushed the boundaries with the introduction of our ODiate Fluorescence Filter Sets, to allow customers to fully optimize their system performance. Our expert engineering team has been involved from the first stage of design to ensure that we exceed your requirements and continues through the entire process of continual production deliveries.

Environmental Stability and Physical Durability

The coating systems used to produce ODiate filters use a dense sputtering process to provide robust environmental reliability to meet the highest demands for thin-film coatings. The resulting highly durable coatings meet or exceed testing procedures including humidity, thermal shock, adhesion, chemical resistance, and moderate abrasion per U.S. Military and international standards.

Specifications

Technical Specs

Type
Fluorescence Excitation Filter
Fluorophores
GFP
Material
BOROFLOAT^® 33
Center Wavelength
474 nm
FWHM
37 nm
Angle of Incidence
0°±5°
Cone Half-angle
<7°
Size
Ø25.0 mm
Size Tolerance
+0.0/-0.1 mm
Thickness
2.0 mm (substrate)
3.5 mm (housing)
Thickness Tolerance
± 0.1 mm
Surface Quality
60-40 scratch-dig

Transmission
Tavg≥96% 458 - 490 nm
Parallelism
≤10 arc seconds
Humidity Resistance
Per MIL-STD-810E, method 507.3, procedure III, modified to 5 cycles
Enhanced Emission Blocking Range
≥ OD10 avg, 505.5 - 541 nm (verified as ≥ OD8 avg)
Blocking Range #1
≥ OD6 avg, 300 - 446.6 nm
Blocking Range #2
≥ OD4 @ 497.8 nm
Blocking Range #3
≥ OD6 avg, 499.8 - 900 nm
Blocking Range #4
≥ OD3 avg, 900 - 1100 nm
Temperature Range
-50 to 100°C

Type
Fluorescence Excitation Filter
Fluorophores
GFP
Material
BOROFLOAT^® 33
Center Wavelength
474 nm
FWHM
37 nm
Angle of Incidence
0°±5°
Cone Half-angle
<7°
Size
Ø25.0 mm
Size Tolerance
+0.0/-0.1 mm
Thickness
2.0 mm (substrate)
3.5 mm (housing)
Thickness Tolerance
± 0.1 mm
Surface Quality
60-40 scratch-dig

Transmission
Tavg≥96% 458 - 490 nm
Parallelism
≤10 arc seconds
Humidity Resistance
Per MIL-STD-810E, method 507.3, procedure III, modified to 5 cycles
Enhanced Emission Blocking Range
≥ OD10 avg, 505.5 - 541 nm (verified as ≥ OD8 avg)
Blocking Range #1
≥ OD6 avg, 300 - 446.6 nm
Blocking Range #2
≥ OD4 @ 497.8 nm
Blocking Range #3
≥ OD6 avg, 499.8 - 900 nm
Blocking Range #4
≥ OD3 avg, 900 - 1100 nm
Temperature Range
-50 to 100°C

Resources & Downloads

Literature

ODiate Fluorescence Filter Sets Datasheet(1.7 MB, PDF)