Fluorescence Excitation Bandpass Filters

Our fluorescence excitation bandpass filters for fluorescence microscopy provide maximum performance using design features including exceptionally high passband transmission, maximized out-of-band blocking including extended range blocking on excitation filters, extremely steep slopes from rejection to transmission regions, high-precision optical glass substrates, and spectral specifications providing maximum image brightness and minimum background.

Enhanced fluorescence emission blocking
Extremely sharp transitions from rejection to transmission regions
Optimized optical fabrication for brilliant imaging

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Description	Compatibility	Price
HPX350-60 Fluorescence Excitation Bandpass FiltersBandpass Filter, Fluorescence, 25.0 mm, 350 nm Center, 60 nm Bandwidth	UNIVERSAL	$306
HPX360-60 Fluorescence Excitation Bandpass FiltersBandpass Filter, Fluorescence, 25.0 mm, 360 nm Center, 60 nm Bandwidth	UNIVERSAL	$150
HPX380-40 Fluorescence Excitation Bandpass FiltersBandpass Filter, Fluorescence, 25.0 mm, 380 nm Center, 40 nm Bandwidth	UNIVERSAL	$306
HPX400-30 Fluorescence Excitation Bandpass FiltersBandpass Filter, Fluorescence, 25.0 mm, 400 nm Center, 30 nm Bandwidth	UNIVERSAL	$150
HPX425-40 Fluorescence Excitation Bandpass FiltersBandpass Filter, Fluorescence, 25.0 mm, 425 nm Center, 40 nm Bandwidth	UNIVERSAL	$306
HPX440-20 Fluorescence Excitation Bandpass FiltersBandpass Filter, Fluorescence, 25.0 mm, 440 nm Center, 20 nm Bandwidth	UNIVERSAL	$150
HPX450-50 Fluorescence Excitation Bandpass FiltersBandpass Filter, Fluorescence, 25.0 mm, 450 nm Center, 50 nm Bandwidth	UNIVERSAL	$306
HPX470-40 Fluorescence Excitation Bandpass FiltersBandpass Filter, Fluorescence, 25.0 mm, 470 nm Center, 40 nm Bandwidth	UNIVERSAL	$150
HPX475-44 Fluorescence Excitation Bandpass FiltersBandpass Filter, Fluorescence, 25.0 mm, 475 nm Center, 44 nm Bandwidth	UNIVERSAL	$150
HPX480-40 Fluorescence Excitation Bandpass FiltersBandpass Filter, Fluorescence, 25.0 mm, 480 nm Center, 40 nm Bandwidth	UNIVERSAL	$150

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Features

Superior Stabilife<sup>®</sup> Coating Technology

HPF™ fluorescence filters are manufactured using our patented Stabilife^® coating technology. They are manufactured using two patented processes for the deposition of metal oxide thin film optical coatings: Reactive Ion Plating (RIP) and Hybrid Plasma Enhanced Deposition (HPED). Both processes yield highly dense, thin film coatings with extraordinary hardness, abrasion resistance, and adhesion to the substrate. Newport's Stabilife processes have been in full-scale production at our coating facility in Franklin, Massachusetts since the early 1990s.

Higher Film Density & Maximized Spectral Stability

For demanding applications such as fluorescence detection, wavelength stability is absolutely critical to insure dependable results. Newport's fluorescence filters with Stabilife coating technology have much higher spectral stability than competitors. One of the critical factor affecting the spectral stability of an optical coating is film density. Unlike competitor's unstabilized metal oxide thick film coating with less film density which typically contributes to environmentally induced spectral shift of 2-5%, Newport's Stabilife coatings have a much higher density and lower void ratio and are therefore less affected by water absorption. They typically exhibit total wet-to-dry shifts of less than 0.02% of wavelength.

Measured transmittance scans of a Stabilife filter at 0% and 100% relative humidity. Scans are exactly overlaid as no shift is discernable at the standard scan speed for a 30nm bandwidth filter.

Minimized Spectral Shifting for Temperature Variations

Stabilife coatings are typically 5 to 10 times less sensitive to thermal variation than un-stabilized metal oxide films as a result of film densification. Temperature change functions are a catalyst for moisture migration in thin films having a significant volume of voids. When un-stabilized films are exposed to high temperatures, moisture migrates out of film voids contributing to the wavelength change discussed earlier. The high film density and reduced permeability resulting from the Stabilife processes reduces this effect providing the maximum spectral stability available for all types of precision coatings including bandpass, dichroic, edge, notch and polarizer coatings.

Measured temperature-induced wavelength shift of an unstabilized metal oxide ultranarrow bandpass filter compared to a Stabilife® ultra-narrow bandpass filter.