In fluorescent up conversion, the fluorescent sample is pumped by a femtosecond pulse and the fluorescence is up converted using an optically gated pulse to generate sum frequency radiation. The up converted light is then spectrally resolved via monochromator or spectrograph using a PMT. The software automatically controls the up conversion crystal’s phase matching angle and monochromator grating angle throughout the fluorescent wavelength range of interest. The software also controls the delay line and gathers time-resolved information which is displayed in a 3D color plot. Calibrated GVD and photometric corrections of spectra are also automated in the software. Compared to pump-probe spectroscopy techniques such as transient absorption, the data interpretation of fluorescence up conversion is straightforward as only emission is measured. Fluorescence up conversion offers fs-resolved dynamics information over a range of 3.3 ns in the UV-VIS and IR spectral ranges.

Applications include:

• Ultrafast dynamics of chemical reactions
• Excited state dynamics
• Conformation dynamics and kinetics in fluorescent molecules
  Quantum dots characterization
• Bioorganic photosynthetic system analysis
• Dynamics in fluorescent proteins and DNA constituents
• Carrier dynamics in nanocrystals and nanoparticles
• Proton transfer, and dye/label photophysics